Age and Sex Differences in the Eμ-TCL1 Adoptive Transfer Mouse Model of CLL

The Eμ-TCL1 model is the most widely used syngeneic mouse of chronic lymphocytic leukemia (CLL) and has been extensively to understand pathogenesis select genes, effect immune microenvironment for pre-clinical drug development studies. Recently there an increasing awareness impact age sex differences on cancers along with efficacy toxicity specific cancer therapies. However, despite predominance older males in CLL patient demographics, adoptive transfer (AT) studies have almost exclusively young female mice. We therefore sought how recipient survival order allow diverse using this model. First, a primary AT experiment was performed assess engraftment survival. found that AT, matched recipients had faster higher burden peripheral blood at 1- 3-months post-AT. while disease correlated survival, prolonged compared male mice regardless donor sex. Notably, were more prominent relatively indolent median 178 132 days respectively as aggressive donors where not apparent both 87 days. A subsequent secondary splenocytes isolated from showed course (median 56 days) which present. Additional experiments determine age. Three cohorts (young 3-6 months) middle aged (9-12 old (18-22 received AT. These ages selected correspond approximate human 20-30 years, 40-45 65 years old. While did significant rate establishment or mice, significantly decreased tumor engraftment, rates failure engraft, but similar overall younger cohorts. In conclusion, we show are present model, having particularly presentations. findings suggest must be considered experimental design adaptive CLL. Figure 1View largeDownload PPTFigure PPT Close modal